Normal Goat Serum from Thermo Fisher Scientific

Product Specs
ItemNormal Goat Serum
CompanyThermo Fisher Scientific
Price Pricing Info Supplier Page View Company Product Page
Catalog Number31872
Quantity120 mg
ApplicationsBlocking assay, Control, Immunocytochemistry, Immunohistochemistry
Formatwhole serum diluted in PBS with 0.05% sodium azide; pH 7.6
Temperature4° C
IsotypeIg
Concentration60.0 mg/mL
SpeciesGoat
DiseaseNormal
Storage4° C

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Thermo Fisher Scientific

81 Wyman Street

Waltham, MA 02451

United States

Phone: 800-678-5599
Fax: 800-331-2286

Company Profile

Website: www.thermofisher.com

(1) TDP-43 pathology in Drosophila induces glial-cell type specific toxicity that can be ameliorated by knock-down of SF2/SRSF1PLOS GeneticsSeptember 25, 2023Sarah Krupp, Isabel Hubbard, Oliver Tam, Gale M. Hammell, Josh Dubnauimaging; or (2) transferred to a blocking solution (10% normal goat serum in 1X PBST; ThermoFisher: 31872) overnight at 4°C on a nutator in preparation for staining. After blocking brains were washed once in

(2) Significance of 8-OHdG Expression as a Predictor of Survival in Colorectal CancerCancers (Basel)September 18, 2023Myunghee Kang, Soyeon Jeong, Sungjin Park, Seungyoon Nam, Jun-Won Chung, Kyoung Oh Kim, Jungsuk An, Jung Ho Kimactivity, and then antigens were electrolyzed. After 30 min of preincubation in 10% normal goat serum (#31872, Invitrogen, Danvers, MA, USA) to prevent nonspecific staining, samples were incubated overnight in

(3) Actionable loss of SLF2 drives B‐cell lymphomagenesis and impairs the DNA damage responseEMBO Molecular MedicineSeptember 11, 2023Le Zhang, Matthias Wirth, Upayan Patra, Jacob Stroh, Konstandina Isaakidis, Leonie Rieger, Susanne Kossatz, Maja Milanovic, Chuanbing Zang, Uta Demel, et al.cells were incubated with 0.3% Triton X‐100 (Sigma, T‐8787) and blocked with 5% goat serum (Invitrogen, 31872) in PBS. The cells were stained with primary anti‐gamma H2A.X (phosphor S139) antibody (Abcam, ab11174)

(4) Mutant IDH regulates glycogen metabolism from early cartilage development to malignant chondrosarcoma formationCell reportsJune 27, 2023Pathmanapan S, Poon R, De Renshaw TB, Nadesan P, Nakagawa M, Seesankar GA, Ho Loe AK, Zhang HH, Guinovart JJ, Duran J, et al.bovine serum albumin (BSA) (Thermo Fisher Scientific, 11020021) & 5% goat serum (Thermo Fisher Scientific, 31872) with Avidin (Vector Laboratories, SP2001) for 1 h. The indicated antibodies and dilutions were used

(5) TDP-43 pathology in Drosophila induces glial-cell type specific toxicity that can be ameliorated by knock-down of SF2/SRSF1bioRxivMay 10, 2023S. Krupp, O Tam, M Gale Gale Hammell, J Dubnauimaging; or (2) transferred to a blocking solution (10% normal goat serum in 1X PBST; ThermoFisher: 31872) overnight at 4°C on a nutator in preparation for staining. After blocking brains were washed once in

(6) Glycation‐mediated tissue‐level remodeling of brain meningeal membrane by agingAging CellMay 1, 2023Hyo Min Kim, Shinheun Kim, Jueun Sim, Boo Soo Ma, Insung Yong, Youngmin Jo, Taek‐Soo Kim, Jae‐Byum Chang, Sung‐Hye Park, Yong Jeong, et al.slices and cells were permeabilized with a blocking solution containing 5% normal goat serum (NGS) (31872, Thermo Fisher Sceintific) in 1% Triton X‐100/1X PBS (1% PBST) solution for over 1 h at RT. Samples

(7) Epigenetic reactivation of tumor suppressor genes with CRISPRa technologies as precision therapy for hepatocellular carcinomaClinical epigeneticsApril 29, 2023Sgro A, Cursons J, Waryah C, Woodward EA, Foroutan M, Lyu R, Yeoh GCT, Leedman PJ, Blancafort P.and washed twice with DPBS. Fixed cells were blocked with 5% normal goat serum (Invitrogen, Cat. No. 31872) and 0.3% Triton X-100 (Sigma-Aldrich) in DPBS for 1 h at room temperature, then incubated with α-Ki-67

(8) Tetrahydrocannabivarin (THCV) Protects Adipose-Derived Mesenchymal Stem Cells (ASC) against Endoplasmic Reticulum Stress Development and Reduces Inflammation during AdipogenesisInternational Journal of Molecular SciencesApril 12, 2023Anna Kowalczuk, Krzysztof Marycz, Justyna Kornicka, Sylwia Groborz, Justyna Meissner, Malwina Mularczykcells were incubated overnight with the Ki-67 antibody diluted in 10% Goat Normal Serum (Invitrogen, #31872, Warsaw, Poland) in PBS at 4 °C (1:1000). Cells were washed with PBS three times and incubated with

(9) Comprehensive Analyses of Muscle Function, Lean and Muscle Mass, and Myofiber Typing in MiceBIO-PROTOCOLFebruary 20, 2023Hima Bindu Durumutla, Chiara Villa, Manoj Panta, Michelle Wintzinger, Ashok Daniel Prabakaran Pragasam, Karen Miz, Mattia Quattrocelli(Table 1) Fluorescently tagged secondary antibodies (Table 2) Goat serum (Invitrogen, catalog number: 31872)Dimethyl sulfoxide (DMSO) (Fisher Scientific, catalog number: D1319)Prednisone powder (Sigma-Aldrich,

(10) Anti-angiogenic collagen IV-derived peptide target engagement with α(v)β(3) and α(5)β(1) in ocular neovascularization modelsiScienceFebruary 17, 2023Lima E Silva R, Mirando AC, Tzeng SY, Green JJ, Popel AS, Pandey NB, Campochiaro PA.Mounting AquaPoly solution,Neobits,Cat # 18606–5 Normal goat serum,Thermo Fisher Scientific,"Cat# 31872, RRID:AB_2532166" Sulfo-SBED Biotin Label Transfer Reagent,Thermo Fisher Scientific,Cat# 33033 Streptavidin (Sepharose Bead Conjugate

(11) Human bone marrow organoids for disease modelling, discovery and validation of therapeutic targets in hematological malignanciesCancer DiscoveryFebruary 6, 2023Abdullah O. Khan, Antonio Rodriguez-Romera, Jasmeet S. Reyat, Aude-Anais Olijnik, Michela Colombo, Guanlin Wang, Wei Xiong Wen, Nikolaos Sousos, Lauren C. Murphy, Beata Grygielska, et al.hours. Immunofluorescence Staining Sections were blocked using 2% goat serum (Thermo Fisher Scientific, cat. #31872) and 1% bovine serum albumin (BSA; Sigma, cat. #A9418) prior to primary antibody labeling with antibody

(12) CD47 restricts antiviral function of alveolar macrophages during influenza virus infectioniScienceDecember 22, 2022Wenzek C, Steinbach P, Wirsdörfer F, Sutter K, Boehme JD, Geffers R, Klopfleisch R, Bruder D, Jendrossek V, Buer J, et al.eFluor 780",ThermoFisher Scientific,65-0865-18 Gentamycin,Sigma Aldrich,G 1272 Goat Serum,Invitrogen,31872 GoTaq® DNA Polymerase,Promega,M3001 Igepal,Sigma Aldrich,I8896 Ionomycin,Sigma Aldrich,I 0634 L-Glutamine,Sigma Aldrich

(13) 4-O-Methylascochlorin-Mediated BNIP-3 Expression Controls the Balance of Apoptosis and Autophagy in Cervical Carcinoma CellsInternational Journal of Molecular SciencesDecember 1, 2022Yuna Cho, Yun-Jeong Jeong, Kwon-Ho Song, Il-Kyung Chung, Junji Magae, Taeg Kyu Kwon, Yung-Hyun Choi, Jong-Young Kwak, Young-Chae Changmethanol and then permeabilized with 0.2% Triton X-100. Cells were blocked with 10% normal goat-serum (#31872, Invitrogen; Thermo Fisher Scientific, Waltham, MA, USA) in PBS. Cells were incubated with LAMP1, LC3,

(14) Morphological Adaptation in the Jejunal Mucosa after Iso-Caloric High-Fat versus High-Carbohydrate Diets in Healthy Volunteers: Data from a Randomized Crossover StudyNutrientsOctober 4, 2022Anna Casselbrant, Ville Wallenius, Erik Elebring, Hanns-Ulrich Marschall, Bengt R. Johansson, Herbert F. Helander, Lars FÃ¤ndrikssections were rehydrated, antigens were retrieved and sections were blocked in 5% normal goat serum (31872, ThermoFisher Scientific, Rockford, IL, USA) before incubation with primary antibodies HMGCS2 (1:150,

(15) Postnatal Conditional Deletion of Bcl11b in Striatal Projection Neurons Mimics the Transcriptional Signature of Huntington's DiseaseBiomedicinesSeptember 23, 2022Song S, Creus Muncunill J, Galicia Aguirre C, Tshilenge KT, Hamilton BW, Gerencser AA, Benlhabib H, Cirnaru MD, Leid M, Mooney SD, et al.Fisher Bioreagents; BP2471-1) and incubated in 5% goat serum (ThermoFisher Scientific, Waltham, MA, USA, 31872) with 0.25% Triton X-100 (Sigma Aldrich, St. Louis, MO, USA, X100-500 mL) in TBS for 1 h at room temperature,

(16) Cannabidiol (CBD) Protects Adipose-Derived Mesenchymal Stem Cells (ASCs) against Endoplasmic Reticulum Stress Development and Its ComplicationsInternational Journal of Environmental Research and Public HealthAugust 31, 2022Anna Kowalczuk, Krzysztof Marycz, Katarzyna Kornicka-Garbowska, Justyna Kornicka, Magdalena Bujalska-Zadrożny, Sylwia Groborzuntreated cells were incubated overnight with the Ki-67 antibody diluted in 10% Goat Normal Serum (#31872, Invitrogen) in PBS at 4 °C (1:1000). Cells were washed with PBS 3 times and incubated with atto-594

(17) Engineered endosymbionts that alter mammalian cell surface marker, cytokine and chemokine expressionCommunications biologyAugust 30, 2022Madsen CS, Makela AV, Greeson EM, Hardy JW, Contag CH.followed by a blocking step containing 0.3% Triton X-100 and 5% normal goat serum (ThermoFisher, cat# 31872,). B. subtilis LLO location was determined by incubating a rabbit anti-subtilisin antibody (1:50, Antibodies-online,

(18) Length-dependent poleward flux of sister kinetochore fibers promotes chromosome alignmentCell reportsAugust 2, 2022Risteski P, Božan D, Jagrić M, Bosilj A, Pavin N, Tolić IM.F2442 Penicillin/Streptomycin,Lonza,Cat# DE17-502E Opti-MEM Reduced Serum Medium,Gibco,Cat# 31985047 Normal Goat Serum,Invitrogen,Cat# 31872 Phosphate Buffered Saline,Roth,Cat# 9143.1 Methanol,Sigma-Aldrich,Cat# 32213-M Formaldehyde,Biognost,Cat#

(19) Sweet taste receptor agonists attenuate macrophage IL-1β expression and eosinophilic inflammation linked to autophagy deficiency in myeloid cellsClinical and translational medicineAugust 1, 2022Lee J, Kim SJ, Choi GE, Yi E, Park HJ, Choi WS, Jang YJ, Kim HS.F(ab′)2‐Alexa Fluor 488 (Jackson ImmunoResearch, 705‐546‐147; 1:250) in PBS with 1% goat serum (Invitrogen, 31872) and 1% bovine serum albumin (Sigma–Aldrich, A9647) for 30 min. Autophagy induction was detected using

(20) Field exposure to 50 Hz significantly affects wild‑type and unfolded p53 expression in NB69 neuroblastoma cellsOncology LettersJuly 5, 2022María Antonia Martínez, Alejandro Úbeda, Javier Martínez‑Botas, María Ángeles Trillowith ethanol/acetic acid for 20 min at −4°C and blocked with PBS containing 10% goat serum (cat no. 31872; Invitrogen; Thermo Fisher Scientific, Inc.) for 1 h at room temperature. Coverslips were incubated

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Normal Goat Serum from Thermo Fisher Scientific

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Normal Goat Serum from Thermo Fisher Scientific

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